Cloning, Gene Expression and Protein Purification: Experimental Procedures and P-image

Cloning, Gene Expression and Protein Purification: Experimental Procedures and P

$ 130.12

Item Width: 10.6 in Publication Name: Cloning, Gene Expression, and Protein Purification : Experimental Procedures and Process Rationale Item Length: 8.2 in Item Weight: 35.9 Oz Item Height: 1 in Number of Pages: 448 Pages gtin13: 9780195132946 Format: Trade Paperback Subject: Life Sciences / Molecular Biology, Life Sciences / Genetics & Genomics, Chemistry / Organic Author: David Presutti, William Miller, Jennifer Edwards, Charles Hardin, Andrew Riell Type: Textbook Book Title: Cloning, Gene Expression and Protein Purification Language: English Publication Year: 2001 Subject Area: Science ISBN: 9780195132946 Country of Origin: United States Publisher: Oxford University Press, Incorporated ISBN-13: 9780195132946

Description

Cloning, Gene Expression and Protein Purification by Charles Hardin, Jennifer Pinczes, Andrew Riell, David Presutti, William Miller, Dominique Robertson Estimated delivery 3-12 business days Format Paperback Condition Brand New Description This combined lecture and laboratory manual presents protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. Publisher Description On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved inisolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoreticalbackground and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins andnucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemicalparticipants, while also giving students the tools needed to become "capable proactive researchers." Features:· Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques· Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations· Discusses fluorophores, and solvent effects on proteinstructure· Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature· Includes carefully selected primary source research literature and articles from current vendorliterature· Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized· Provides an alphabetized list of common reagents for rapid reference· Offers an extensive index of concepts and terms· Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and AlternativeApproaches Details ISBN 0195132947 ISBN-13 9780195132946 Title Cloning, Gene Expression and Protein Purification Author Charles Hardin, Jennifer Pinczes, Andrew Riell, David Presutti, William Miller, Dominique Robertson Format Paperback Year 2001 Pages 448 Edition 1st Publisher Oxford University Press Inc GE_Item_ID:137021721; About Us Grand Eagle Retail is the ideal place for all your shopping needs! With fast shipping, low prices, friendly service and over 1,000,000 in stock items - you're bound to find what you want, at a price you'll love! Shipping & Delivery Times Shipping is FREE to any address in USA. Please view eBay estimated delivery times at the top of the listing. Deliveries are made by either USPS or Courier. We are unable to deliver faster than stated. International deliveries will take 1-6 weeks. NOTE: We are unable to offer combined shipping for multiple items purchased. This is because our items are shipped from different locations. Returns If you wish to return an item, please consult our Returns Policy as below: Please contact Customer Services and request "Return Authorisation" before you send your item back to us. Unauthorised returns will not be accepted. Returns must be postmarked within 4 business days of authorisation and must be in resellable condition. Returns are shipped at the customer's risk. We cannot take responsibility for items which are lost or damaged in transit. For purchases where a shipping charge was paid, there will be no refund of the original shipping charge. Additional Questions If you have any questions please feel free to Contact Us. Categories Baby Books Electronics Fashion Games Health & Beauty Home, Garden & Pets Movies Music Sports & Outdoors Toys

  1. Like new condition, safe packaging, good communication from seller. Fair price for the quality. Shipping was on time, but tracking information took over a week to receive. The book was as-described with minor cosmetic flaws (indentations in some pages, for example). I wish the listings showed the actual item being sold to you instead of just an accurate stock photo. This edition and appearance of George Orwell's 1984 here is a nice pocket-sized hardcover. I would buy from this shop again.

    JeffsHouse15

  2. The two new out-of-print books I ordered were exactly as described and shipped promptly. I did ask the seller's support person (an AI chatbot, I think) to be sure to package the items carefully, and "he" said that he'd make that request of the shipping department. I knew this was hooey, and as I expected, the books were shipped in nothing but a padded paper mailer, no other protection. I got lucky, no damage, but don't bother making special requests of this seller.

    Olivier Marguillard

  3. Lightning fast shipping. Item arrived before the delivery date, perfect. Item arrived carefully packaged and was exactly as described. Very pleased.  Great communication throughout the process. Very attentive to detail and responsive. Will buy from again!  Had a small issue with the item, but the seller resolved it quickly. Great customer service!  The seller went above and beyond to make sure I was happy with my purchase. Highly recommended!

    Anthony04357

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